The layoffs are part of a plan to save $5.5 billion and make the Disney+ streaming service profitable, which has reported a sharp decline in subscribers since 2019.
He also released the first set of financial results since he returned to the company in November, revealing ongoing losses for Disney+.
Announcing the job cuts, he said: “I have great respect and appreciation for the talent and dedication of our employees around the world, and I recognize the personal impact of these changes.”
The job cuts represent about 3.6% of Disney’s global workforce. Meanwhile, Disney also reported an 8% rise in sales to $23.5bn (£19.45bn) between October and December last year. Profits also rose 11% to $1.3 billion.